Division of Fish Genetics and Biotechnology ( FGB)

9697885899
ahmadirfan@skuastkashmir.ac.in

S.No

Name of Student

Reg.No

Masters Research Topic

Date

Abstract & Key Words

01

Asim Iqbal Bazaz

(MFSc)

2016-F-52-M

Induction of triploidy using heat shock treatment in trout of Kashmir Himalaya

2019

Triploidy was successfully induced in

Oncorhynchus mykissusing heat shock treatment. For inducing triploidy a total of 15 healthy parent stocks of male and femaleOncorhynchus mykissin the ratio of 2:1 were taken for experimentation. Shortly after fertilization, triploidy was induced by thermal (heat) shock treatment to the fertilized eggs at 28oC and 30C for 10 minutes, 10 and 12 minutes after fertilization. In control group the fertilization rate of 86.60±1.24%, hatching rate of 74.44±0.88% and percentage at yolk sac absorption of 71.61±0.12% was observed. Among treatment groups, the highest fertilization percentage of 76.67±1.24% was found at a heat shock of 30C, 12 minutes after fertilization. Highest hatching percentage and maximum percent survival at yolk sac absorption of 64.32±0.94% and 60.56±0.44% respectively was observed at a heat shock treatment of 28oC beginning 12 min after fertilization for duration of 10 min. Induction of triploidy was confirmed by karyotyping analysis on trout fry. The highest triploidy rate of 35.00±5.00% was found at a heat shock of 30oC, 12 minutes after fertilization for duration of 10 minutes.

Keywords:

Triploidy ,  Chromosomes, fertilization, karyotyping,

Oncorhynchus mykiss

 

02

ZarkaYousuf

(MFSc)

2016-F-46-M

Genetic variation in Schizothorax curvifrons(Heckel, 1838)along the trophic gradients of Kashmir Valley

2019

The present study was aimed to evaluate genetic variability in

Schizothorax curvifrons

from two lakes of Kashmir valley namely Dal lake, located in district Srinagar  and Manasbal lake, located in Safapora, district Ganderbal having different trophic gradients using biochemical genetic marker. The samples were collected from the landing centres of the respective areas. A total of 180 samples were collected after every 10 days for a period of 4 months from December 2017 to March 2018. SDS-PAGE analysis showed a total number of 17 bands from both populations. 8 bands of Molecular weights ranging from 10 to 150 kDa from Dal were revealed and 9 bands of Molecular weights ranging from 15 to 200 kDa were resolved. The sampled

Schizothorax curvifrons

populations from the Dal Lake showed three common bands on the gel with the sampled

S.curvifrons

populations of Manasbal Lake. The common bands had molecular weights of 75 kDa, 25 kDa and 15 kDa. The Rf values of the Manasbal Lake population ranged from 0.07 to 0.69. Similarly in Dal lake population, the Rf values ranged from 0.14 to 0.77. This study provides information about the distribution and patterns of genetic variability at intraspecific population level of

Schizothorax curvifrons

. This study also reveals the degree of variation in the electrophoretic patterns of muscle proteins. The present study documents the protein fraction variations at trophic gradients as genetic clines.

 

Key words:

Schizothorax,

SDS-PAGE, Molecular Weight, Rf, Genetic marker.

 

03

Nafhatul Arab

(MFSc)

2016-F-40-M

Assessment of genetic variation in Schizothorax  esocinus from Dal and Manasbal Lakes of Kashmir Himalaya

2019

Schizothorax esocinus

(Churru snow trout) Heckel, 1838 is a key freshwater specie in the valley of Kashmir. The present study was aimed to evaluate the genetic variability in the fish from the two eminent lakes of Kashmir valley namely Dal and Manasbal lakes having different trophic gradients using biochemical (genetic) marker (SDS-Page). The electrophoretic analysis revealed 6 bands of Molecular weights ranging from 13 to 150 kDa for Dal Lake and 8 bands of Molecular weights ranging from 15 to 150 kDa for Manasbal Lake. The Rf value ranged from 0.15321 to 0.70625 for sampled fishes of Dal Lake and from 0.14231 to 0.63559 for sampled fishes of Manasbal Lake. The study provided some basic information about the genetic variation of

S. esocinus

populations in the Dal and Manasbal lakes. Electropherogram studies revealed that the studied populations of

Schizothorax esocinus

show some degree of variation in the electrophoretic migration of muscle proteins. Polymorphism in muscle protein is clearly demonstrated among this group of fishes from the two lakes.

Keywords:

Schizothorax

, SDS-Page, Molecular Weight, Protein, Rf, Genetic markers.

04

MehakHafeez

(MFSc)

2017-F-64-M

Virulence gene profiling of Clostridium perfringens from fish and aquatic environment of Dal lake

2019

During the current study, the presence of different toxinotypes of

Clostridium perfringens

in the icthyofauna particularly

Cyprinus carpio

var.

communis

(Scale-carp) and

Schizothorax niger

(Snow-trout) and the aquatic environment of the Dal Lake was investigated. A total of 165 samples (60 each of scale-carp and snow-trout; 45 water), collected from different sites of Dal Lake, were screened for the presence of

C. perfringens

. Using species-specific 16S rRNA gene amplification, all the 45 (100%) water samples and 37 (30.8%) of 120 fish samples were found to be positive for

C. perfringens

. The toxinotyping of

C. perfringens

isolates carried out by multiplex-PCR targeting six toxin genes (

cpa, cpb

,

εtx, ιtx, cpb

2and

cpe

) revealed that all the 45 isolates from water and 37 isolates from fish (21 from scale-carp and 16 from snow-trout) were carrying

cpa

gene and thus were designated as toxinotype A. None of the isolates carried other toxin genes like

cpb

,

εtx

,

ιtx

,

cpb

2or

cpe

. It was also observed that the prevalence of

C. perfringens

was slightly higher in scale-carps (35%) compared to snow-trouts (26.6%). During the present study, the bacterial load of

C. perfringens

at five different sites of the Dal Lake was calculated and was found to be in the range of 9.2 x 10 CFU/ml to 1.87 x 10

2

CFU/ml, being lowest at Char-Chinari and highest at Dalgate, which is less than the critical CFU level of

C. perfringens

. Antibiotic sensitivity test of

C. perfringens

type A isolates revealed highresistance to more than 3 antimicrobials with amoxicillin/clavulanic acid, chloramphenicol and ciprofloxacin as being most sensitive. The

cpa

toxin genes of

C. perfringens

type A isolates obtained from water and fish sources during the study were sequenced and the outline of phylogenetic tree revealed 95% to 98% homology with corresponding GenBank published sequences. The phylogenetic analysis of the protein sequences of

cpa

toxin revealed a close relationship with corresponding AA sequences of

C. perfringens

strains reported from India, Egypt and China. The present study documented the presence of

C. perfringens

toxinotype A in fish and water for the first time in the Dal Lake of the Kashmir valley and implied that fish may be a possible source of

C. perfringens

type A intoxication to humans through food chain.

Key words:

C. perfringens

, fish, 16S rRNA, toxins, subtyping, multiplex-PCR, CFU, drug-resistance, phylogeny, public health.

 

05

Sumaira Rashid

(MFSc)

2017-F-63-M

Detection of Cyp.P450 in Fish- A Potential biomarker of Aquatic Environmental Contamination

2019

The present study was carried out to detect and quantify cytochrome P4501A in

Cyprinus carpio

var.

communis

collected from Dal lake. Physic-chemical parameters, viz., Water temperature, pH, Total Phosphorus, Ammonical Nitrogen and Nitrate Nitrogen were also monitored during the study. Samples were collected from two different sites of the Dal lake viz, Nishat and Hazratbal basin and experimental control from December 2018 to March 2019. Samples were collected on monthly basis. Cytochrome P4501A was detected in all the samples examined throughout the study.Using one way analysis of variance, P<0.01 was obtained which indicated that there was a significant difference (P<0.05) in CYP1A levels among the 3 sampling sites viz., Hazratbal, Nishat and control. Further, it was observed that CYP1A showed the positive correlation with both total phosphorus (r=0.30, P>0.05) as well as nitrate nitrogen (r=0.07, P>0.05). Both CYP1Alevel as well as the total phosphorus and nitrate levels was highest in samples collected from Hazratbal basin and lowest in the samples collected from control. Limnological parameters were found in the range of: total phosphorus (86-746µg/L), nitrate nitrogen (80-387µg/L), water temperature (7-12.3 ºC), pH (7-7.7), ammonical nitrogen (46-444µg/L).

Key words:Cytochrome P450, Dal Lake,

Cyprinus carpio

, Total Phosphorus, Nitrate nitrogen.

06

Hussna

(MFSc)

2017-F-65-M

Assessment of Growth performance by RNA:DNARatio in Cyprinus carpio. under varied dietary protein levels

2019

The present growth trial was conducted on scale carp fingerlings (

Cyprinus carpio

var.

communis

) fed with three different levels of silkworm pupae supplement [17.51 g/100g (T

1

), 14.42 g/100g (T

2

), 11.33 g/100g (T

3

)] with a control in which no silkworm pupae was included. The aim of the study was to determine the RNA: DNA ratio as an indicator of growth performance in scale carp after fed with dietary protein level . RNA:DNA ratio were  highest in T

1

(1.609), followed by T

2

(1.526) and T

3

(1.239) while the control showed the lowest ratio (0.929). Similarly fingerlings fed with Silkworm pupae supplemented diets (T

1

, T

2

and T

3

) exhibited relatively better growth in terms of body weight gain, percent  weight gain, specific growth rate (SGR), feed conversion ratio (FCR) and protein efficiency ratio (PER) as compared to the control . The highest weight gain of 2.4 g was recorded in T

1

followed by T

2

(2.09 g) and T

3

(1.52 g) while the control achieved the lowest weight gain of 0.72 g. Overall, the results  revealed that T

1

performed better as compared to the rest treatments and control. In general, the RNA: DNA ratio and growth results correlate with each other in different treatments and control. So, RNA: DNA ratio confirms to be as an effective and rapid indicator of growth performance. 

Key words: RNA:DNA ratio, silkworm pupae, growth, protein.

07

SaimaAndleeb

(MFSc)

MSFy/2018/83

Detection of cytochrome P450 in

Carassius carassius

from Ahansar Lake

 

2021

The present investigation was carried out at the Fish Genetics and Biotechnology Laboratory, Faculty of Fisheries, SKUAST-K, Rangil, Ganderbal from December 2019- February 2020 to detect Cytochrome P4501A (CYP1A) in

Carassiuscarassius

collected from Wular Lake. The samples were collected on monthly basis from three different sites of the lake. CYP1A was detected in all the samples examined throughout the study period. Using one way analysis of variance, P < 0.01 was obtained which indicated that there was a significant difference (P<0.05) in CYP1A levels among the 4 sampling sites viz., Kulhama, Laherwalpora  and Ashtung and control group. There was the +ve significant correlation (r = 0.94, P<0.01) between total phosphate and nitrate. Further, it was observed that CYP1A showed the +ve correlation with both phosphate (r= 0.89, P< 0.01) as well as nitrate concentrations ( r= 0.88, P< 0.01). In the present study, CYP1A concentration was studied in relation to the total phosphorous and nitrate levels at the respective sites. Both CYP1A level as well as the phosphate and nitrate levels was highest in samples collected from Site B (Laherwalpora) and lowest in the samples collected from control group. It was evident from the present study that with the increase in phosphate and nitrate content, CYP1A levels also increased considerably. In other words the two parameters i,e phosphate and nitrate concentration in water showed a direct relationship with CYP1A concentration in fishes.

 

Key words: Cytochrome P4501A, Wular Lake,

Carassiuscarassius

, Total phosphorus, Nitrate nitrogen.

 

08

SheebaZahoor

(MFSc)

MSFy/2018/82

Expression of Cytochrome P450 in common carp from             Manasbal Lake

 

2021

Manasbal Lake is an important water body for the local residents of Yangoora & Safapora towns of district Ganderbal. It is polluted by domestic wastewaters together with natural contamination and pesticides, which have had cumulatively negative effects. The present study was carried out to detect and quantify cytochrome P4501A in

Cyprinus carpio

var.

communis

collected from Manasbal lake. The general water physico-chemical parameters like Water temperature, pH, Dissolved Oxygen, Ammonical Nitrogen, Nitrate Nitrogen and Total Phosphorus, were measured at each sampling sites during each fish sampling period. A total of 180 fish samples ranging from 30g to 90 g in total weight were collected from three different sites of the Manasbal lake viz., Main Ghat, Jarokabagh, and Kondabal and from experimental control from December 2019 to February 2020, taking 15 samples per month from each site. Hepatic CYP1A quantities amongst the sites was assessed by Competitive ELISA. Cytochrome P4501A was detected in all the samples examined throughout the study. Using one way analysis of variance, P<0.01 was obtained which indicated that there was a significant difference (P<0.05) in CYP1A levels among the 4 sampling sites viz., Main Ghat, Jarokabagh, Kondabal and Control. Further, it was observed that CYP1A showed the positive significant correlation with both total phosphorus (r=0.94, P<0.01) as well as nitrate nitrogen (r=0.95, P<0.01). Both CYP1A level as well as the total phosphorus and nitrate levels was highest in samples collected from Kondabal and lowest in the samples collected from control indicating the former to be the most polluted site as compared to others. Limnological parameters in the range for total phosphorus (86.2-351.5 μg/L), nitrate nitrogen (74.4-295.4 μg/L), ammonical nitrogen (57.6-146.2 μg/L) dissolved oxygen (7.1-9.8 mg/L), water temperature (3.3-11.9 ºC), and pH (7-7.8) was found. The study shows that the induction of the biomarker, Cytochrome P4501A1 might be useful for the assessment of environmental contamination in the Manasbal Lake (Ganderbal, J&K).

 

Key words:      

Cytochrome P450,

Cyprinus carpio,

Manasbal Lake, ELISA, Total phosphorus, Nitrate nitrogen.